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Grayburn has several projects that are available for undergraduate participation. Opportunities are available for undergraduate students to obtain hands-on research in a molecular biology lab. In this assay, PCR products are labeled with fluorescent dyes that can be detected using a Beckman Coulter CEQ 8000 Genetic analysis system. It is possible to distinguish individual plants, snakes, or other creatures from each other. The Molecular Core Lab uses capillary electrophoresis for DNA genotyping analysis. A Mx3000P instrument from Agilent Technologies is available to perform these reactions. Many studies in different organisms are possible with this technology. It can also be used to investigate how a gene deletion affects the expression of other genes. This procedure has been used to determine if specific genes are expressed at different levels in response to growth conditions or stage of developmental.
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Gene expression studies frequently use quantitative real-time PCR (qPCR). Centrifuges, thermal cyclers, lyophilizers, autoclaves, ovens, a dishwasher, and gel documentation equipment are available for student use. Grayburn has been working in labs with nucleic acids since 1981 and can help students with many aspects of molecular biology.
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The main focus of the Molecular Core Lab is support for purification of DNA and RNA and the use of molecular biology equipment for analysis of gene expression and genotyping.